mgieasy environmental microbiome dna extraction kit  (Complete Genomics Inc)

Journal: Frontiers in Microbiology

Article Title: Multi-omics reveals glutinous rice varieties shape Baijiu flavor via microbial and metabolic modulation

doi: 10.3389/fmicb.2025.1721127

Figure Lengend Snippet: Metagenomic analysis of microbial communities in Zaopei. (A) Stacked bar chart of species-level relative abundances showing the top 30 most abundant taxa; others were grouped as “Other,” and kingdom-level classification. (B) Alpha diversity indices represent species richness and evenness. (C) Heatmap of significantly different functional categories. Non-parametric statistical tests (Wilcoxon/Kruskal–Wallis) were applied, and the top 30 categories with p < 0.05 were visualized. (D) LDA score plot based on LEfSe analysis, indicating taxa with significant differences among groups; the x -axis shows LDA scores, and the y -axis lists discriminatory taxa. (E) Principal component analysis (PCA) plot with ANOSIM test assessing group-level differences. (F) Venn diagram of differentially abundant species. (G) Boxplot of beta diversity illustrating within-group variability.

Article Snippet: Total microbial DNA was extracted from Zaopei samples collected on day 8 of fermentation, with three biological replicates for each sample, using the MGIEasy Microbiome DNA & RNA Extraction Kit (MGI Tech, China).

Techniques: Functional Assay

Journal: Endocrine Connections

Article Title: Rapid and selective gut microbiome modulation by polyherbal formulation in type 2 diabetes

doi: 10.1530/EC-25-0463

Figure Lengend Snippet: CCA and taxonomic composition of the gut microbiome. (A) CCA of gut microbiome profiles in PHF-treated patients (salmon) and the placebo group (pale green) before and after the 7-day intervention. Time points are distinguished by shape: circles indicate the baseline (before intervention) and triangles indicate the post-intervention period. The colored ellipses represent grouping by study arm (PHF or placebo) and time (samples collected pre- or post-intervention). The arrows represent the centroids of the constrained factors (arm and time) in the CCA model. Only arm was identified as a significant contributor to microbiome variation by PERMANOVA; time is shown for visualization. (B) Taxonomic composition of the 20 most abundant bacterial species across all samples, shown as relative abundances in stacked bar plots. The samples are grouped by study arm and time (placebo group before intervention, placebo group after intervention, polyherbal formulation group before intervention, and polyherbal formulation group after intervention), with taxa ordered by the mean abundance within each group. CCA: canonical correspondence analysis; PHF: polyherbal formulation.

Article Snippet: DNA extraction was performed with the MGIEasy Stool Microbiome DNA Extraction Kit on the MGISP-960 automated platform (MGI Tech Co., Ltd, China).

Techniques: Formulation